Comprehensive suite of molecular biology and sequence analysis tools

Explore a list of the comprehensive set of molecular biology and sequence analysis tools available in Geneious Prime
NGS Pre-Processing
Import Illumina, PacBio and NanoPore reads
Trim, filter and demultiplex both single-end and paired-end data
Merge paired reads
De-duplicate
Error correct and normalize
Filter out chimeras
Learn more about NGS pre-processing
Mapping and de novo Assembly
Simply switch between industry leading algorithms for mapping and de novo assembly
Support for assembly of Sanger and NGS data, including Illumina, PacBio and Oxford Nanopore reads of any length, including paired-end reads and hybrid assemblies
Produce circular contigs when assembling microbial genomes, plasmids and other circular sequences
Genome comparison and finishing with MAUVE genome alignment
Mappers including Geneious, Geneious for RNA Seq, BBMap, Minimap2, Bowtie2 and TopHat
De novo assembly algorithms including Geneious, SPAdes, Flye, MIRA, Tadpole and Velvet
Learn more about mapping and de novo assembly
Variant Calling and Expression Analysis
Call SNPs/variants using Geneious or FreeBayes
Perform real-time filtering of tabular results with synchronized genome view
Calculate and compare expression levels on mapped RNA-seq data
Visualize using PCA and volcano plots
Learn more about NGS analysis
Sequence Analysis
Trim, assemble, and view Sanger sequencing trace files
Correct base calls and create consensus sequences
Annotate motifs, ORFs and repeats
Predict genes and structural elements
Real-time annotation via similarity search against database
Translate selections on the fly, or show translation for annotations or selected frame
Dynamic graphs and statistics for sequence properties such as pI, molecular weight, melting point, AA composition and more
Learn more about Sanger sequence analysis
Sequence Alignment
Multiple and pairwise sequence alignment of DNA or protein including full genome alignment
Align with trusted algorithms including Geneious Aligner, MUSCLE, MAFFT, Clustal Omega, MAUVE and LastZ
View and edit alignments with real-time translation and highlighting
Learn more about alignment
Phylogenetics
Build trees with Geneious tree builder, MrBayes, PAUP*, PhyML, RAxML and more
Visualize, edit and markup your trees
Interactive distance matrix viewer
Publication quality export
Learn more about phylogenetic tree building
Microsatellite Analysis
Import raw ABI trace files
Trim, predict and manually adjust peaks
Bin peaks into alleles
Produce tabular output of allele calls
Learn more about microsatellite analysis
Molecular Cloning
View plasmid maps, automatically annotate vectors, and copy-paste sequences with annotations
One-step GoldenGate (Type IIS) and Restriction cloning
Homology-based cloning including Gibson, GeneArt, and In-Fusion
TOPO cloning
Parent/descendant lineage tracking of cloning operations
Codon optimization and back translation
Silent mutation analysis to find potential restriction sites to introduce
CRISPR gDNA design
Simulated PCR, digestion and ligation
Learn more about cloning
Primer Design
Automatically design PCR and sequencing primers and hybridization probes, to any target region or entire sequence
Easily add primers in the Sequence View
Design basic and degenerate PCR primers
Add and remove extensions to a primer sequence before, during or after the design process
Primer specificity testing to check for additional binding sites on the template sequence
Screen for physical properties, hairpins and primer-dimers
Drag and drop your primers in FASTA, spreadsheet or GenBank format
Learn more about primer design
Data Management and Collaboration
Drag and drop import of files and folders, including Vector NTI databases
Import metadata from a spreadsheet onto sequences and other documents
Smart NGS import – one step import of any assortment of SAM, BAM, GFF, BED, and VCF files
Intuitive folder-based project organization
Seamlessly integrated shared database
Rapid search against all sequences and metadata in your database
Extensive export options
Learn more about data management
Searching and BLAST
Direct access to NCBI public BLAST databases
Custom BLAST for private local databases
Integrated search of external databases including GenBank and UniProt
Upload your sequences directly to GenBank
Search for literature in PubMed
Advanced searching against your local or shared database
Learn more about searching and BLAST
Automated Workflows
Create workflows for automated bulk analysis using a visual editor
More than 20 in-built workflows for performing pipelines including Apply Variants to Reference Sequence, Map Reads then find SNPs, and Randomly Sample Sequences
Extend capabilities with option to write custom code workflows
Learn more about workflows
API & Developers
Add specialized functionality or integrate with other systems using the Plugin Development Kit
Add your favorite algorithm, database or visualization
Wrap a command line program to run via the Geneious Prime GUI

Product: Geneious Prime 2021.1
Version:2021.1
Supported Architectures: x64
Language: english
Supported Operating Systems:Windows 7even or newer
Size: 1CD